Diacetyl | Definition

Doc's Brewing Glossary by Adam McKee

Diacetyl is an organic compound produced during fermentation that can impart a buttery or butterscotch flavor to beer.

Understanding Diacetyl

Diacetyl (2,3-butanedione) is a natural byproduct of yeast metabolism during fermentation. While it is present in most beers at low levels, concentrations above the flavor threshold (around 0.1 parts per million) can result in a distinct buttery or butterscotch aroma and flavor, which is generally considered undesirable in most beer styles.

Diacetyl Formation and Reduction

  • Formation: During fermentation, yeast produces alpha-acetolactate, a precursor to diacetyl. This precursor is then oxidized into diacetyl, which is released into the beer.

  • Reduction: Healthy yeast cells have the ability to reabsorb diacetyl and reduce it into acetoin and 2,3-butanediol, compounds that have little to no flavor impact on the beer. This reduction process typically occurs towards the end of primary fermentation and can be accelerated through a diacetyl rest.

Diacetyl Rest

A diacetyl rest is a common brewing practice used to reduce diacetyl levels in beer. It involves raising the fermentation temperature slightly for a short period, usually towards the end of primary fermentation. This temperature increase stimulates the yeast’s metabolism and encourages it to reabsorb and reduce the diacetyl present in the beer.

Causes of Elevated Diacetyl Levels

Several factors can contribute to elevated diacetyl levels in beer:

  • Yeast Strain: Some yeast strains are more prone to producing diacetyl than others. For example, certain lager yeast strains are known for higher diacetyl production.

  • Fermentation Temperature: Fermenting at temperatures below the optimal range for a particular yeast strain can slow down yeast activity and hinder the reduction of diacetyl.

  • Nutrient Deficiencies: Insufficient nutrients, such as zinc or valine, can impair the yeast’s ability to reabsorb and reduce diacetyl.

  • Bacterial Contamination: Certain bacteria, such as Lactobacillus and Pediococcus, can produce diacetyl.

Diacetyl in Different Beer Styles

While diacetyl is generally considered a flaw in most beer styles, some styles, such as traditional English ales, may have low levels of diacetyl that are considered acceptable or even desirable. However, most brewers strive to minimize diacetyl levels in their beer to achieve a clean and balanced flavor profile.

Preventing and Reducing Diacetyl

To prevent or reduce diacetyl in your beer:

  • Choose the Right Yeast: Select a yeast strain that is known for low diacetyl production, especially for lager styles.

  • Pitch Adequate Yeast: Ensure you are pitching enough healthy yeast cells to carry out a vigorous fermentation.

  • Maintain Proper Fermentation Temperature: Follow the recommended temperature range for your chosen yeast strain.

  • Provide Sufficient Nutrients: Make sure the wort contains enough nutrients to support healthy yeast growth and metabolism.

  • Consider a Diacetyl Rest: If you are using a yeast strain prone to diacetyl production or if you detect a buttery flavor in your beer, a diacetyl rest may help to reduce the diacetyl levels.

Conclusion

While diacetyl can be a nuisance for brewers, understanding its causes and implementing preventive measures can help you avoid this off-flavor and produce clean, delicious beer. By paying attention to yeast selection, fermentation temperature, nutrient levels, and sanitation practices, you can minimize diacetyl formation and ensure that your beer is free from any unwanted buttery notes.

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